GSM6610464: V. cholerae Δtgt + tobramycin_replicate 3 [tgttob3_S51]; ... - SRA

SRX17761044: GSM6610464: V. cholerae Δtgt + tobramycin_replicate 3 [tgttob3_S51]; Vibrio cholerae O1 biovar El Tor str. N16961; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 24.1M spots, 1.7G bases, 554.9Mb downloads

External Id: GSM6610464_r1

Submitted by: Plasticité du Génome Bactérien, Génomes et Génétique, Institut Pasteur

Study: V. cholerae transcriptome or deleted for the tgt gene in MH, supplemented or not with tobramycin or ciprofloxacin

PRJNA885798 • SRP400425 • All experiments • All runs

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We study gene expression levels of V. cholerae N16961 WT and tgt mutant strains in MH medium in the presence or not of sublethal concentration on tobramycin or ciprofloxacin Overall design: Bacterial cells were grown to OD 0,3 and RNAseq was performed.

Sample: V. cholerae Δtgt + tobramycin_replicate 3 [tgttob3_S51]

SAMN31104804 • SRS15288406 • All experiments • All runs

Organism: Vibrio cholerae O1 biovar El Tor str. N16961

Library:

Name: GSM6610464

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cultures were centrifuged, and RNA was harvested using Trizol reagent. Illumina TruSeq mRNA Sample Prep Kit (20020595) was used with 7 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols (Krin et al., 2018, PMID: 29783948)

Runs: 1 run, 24.1M spots, 1.7G bases, 554.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR21765952	24,077,115	1.7G	554.9Mb	2022-10-03

ID:: 24669244

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